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首頁(yè)-技術(shù)文章-CEM微波合成用于γ-Lactam的研究

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CEM微波合成用于γ-Lactam的研究

更新時(shí)間：2012-06-29       點(diǎn)擊次數(shù)：2736

INTRODUCTION

One challenge facing peptide synthesis is the -lactam formation of arginine （Figure 1）. The formation of -lactam occurs when the internal nitrogen of the guanidine attacks the activated ester, irreversibly forming the lactam ring. This reaction is competitive with peptide-bond formation, greatly reducing the coupling efficiency of arginine.

Figure 1: γ-Lactam Formation

The goal of this study was to determine what effect microwave irradiation has on -lactam formation and how to optimize the microwave synthesis of arginine-containing peptides. As a test substrate, we selected the 1992 ABRF study peptide sequence. This sequence is known to suffer from Arg deletion due to -lactam formation.

 
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